FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004954747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004954747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29856287
Sequences flagged as poor quality0
Sequence length30
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1800240.602968480307012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAAAAG733000.020.6960287
GTAGGGA92400.017.3973662
AAAAAGG911650.016.2076768
AAAGGGG914750.015.89622110
TAGGGAA98900.015.8749823
GGTAGGG106700.015.3787821
AAGGGGG955650.015.36931211
AAAAGGG841950.015.3090069
GTGGGGA311750.014.9420992
TGGGGAA285700.014.5340533
AAAGGAG163000.013.83490610
AGGGAAA215350.013.1687954
AGGGGGG1325350.013.11494412
GTGGAAA135850.012.94982922
AAGGAGG174950.012.835211
GGTGGAA123750.012.55767321
GAAAGAG142600.012.2017937
GGAAAGA170100.011.9417546
GGGGAGA359850.011.6678274
AGAAAGG156050.011.4665878
Produced by FastQC (version 0.11.5)