FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004954828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004954828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences612203
Sequences flagged as poor quality0
Sequence length40
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCATCT9442315.423478813400129TruSeq Adapter, Index 1 (97% over 37bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCATCT24150.39447699537571684TruSeq Adapter, Index 18 (97% over 36bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCATCT14680.2397897429447422TruSeq Adapter, Index 18 (97% over 36bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCATCT12520.20450732845151035TruSeq Adapter, Index 18 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)