FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004954996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004954996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23138049
Sequences flagged as poor quality0
Sequence length36
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1689890.7303511199237239No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGTTTTT371800.16068770534628912No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGTTTT313640.13555161889405629No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCCG39800.018.6810593
AAAGTTT293800.017.98056628
AAAAGTT295000.017.89120527
GAAAAAG315800.017.10206825
AAAAAGT321600.016.79323626
AAGTTTT263600.016.5931429
TTCCGAT44150.016.506965
AAAGGTT152850.016.42690828
GGGGGAG525550.016.1979753
AAAAGGT158000.016.10894427
AGTTTTT287300.015.52263630
GGGAAAA809950.015.46449523
AAGGTTT138700.015.34590129
GGGGGAA843050.015.23172821
CTTCCGA47750.015.1970854
GGGAGAG194000.015.1114655
TCCGATC48600.014.997376
GGGGAAA857700.014.988564522
GGAAAAA797400.013.93918124
GGGGAGA217600.013.9112374
Produced by FastQC (version 0.11.5)