FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004955046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23831039
Sequences flagged as poor quality0
Sequence length36
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1651380.6929534209565936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCCG30250.015.8070143
AAAGTTT193300.013.18335628
AAAAGTT197900.013.11115727
TTCCGAT36400.012.8941975
GGGGGAA510150.012.798923521
AAAGGTT104200.012.73913628
GGGAAAA508050.012.65327223
GGGGAAA518750.012.42723722
AAAAGGT111650.012.10338327
CTTCCGA39700.012.0094
GAAAAAG233050.011.81595525
GGGGGAG370100.011.7169323
AAAAAGT233800.011.66221326
GGAAAAA535450.011.42275424
AAGTTTT184650.011.25877429
AAGGTTT100100.010.69895629
AGTTTTT203600.010.4987630
GGGAGAG164450.09.3372165
TCCGATC50650.09.3271126
GGGGAGG322800.09.1356844
Produced by FastQC (version 0.11.5)