FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004955049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19459121
Sequences flagged as poor quality0
Sequence length40
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTC1709100.8783027763689839TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA194700.032.3351252
GATCGGA190850.031.9883421
ACACGTC196350.031.43673713
CGTCTGA199100.030.98433916
ACGTCTG200450.030.80126215
CACACGT201600.030.59411212
TCGGAAG208050.030.3829233
CACGTCT205450.030.09310514
GCACACG210000.029.43511211
CGGAAGA215800.029.2208794
GTCACAT209900.029.1634129
AGCACAC217650.028.51768510
AGAGCAC219200.028.3862698
GTCTGAA219400.028.16399617
GAGCACA222550.027.9434159
TCTGAAC221400.027.8711918
ACATTCC224200.027.33355732
CCAGTCA224800.027.29869526
CAGTCAC226750.027.13876227
AGTCACA226700.027.11468328
Produced by FastQC (version 0.11.5)