FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004955050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19459121
Sequences flagged as poor quality0
Sequence length36
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG988700.5080907816956377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT30250.015.3175035
TCCGATC31850.014.6442936
CCGATCT34650.012.5538517
CTTCCGA40050.011.9792244
AAGTTGG58350.011.92783329
TCTTCCG42150.011.2377743
AAAAGTT116250.010.9921627
GGGAAAA288300.010.89853223
AAAGTTG69050.010.86099328
GGGGGAA299150.010.55921121
GGGGAAA306000.010.33711622
AAAAAGT136450.010.29901726
GGAAAAA306550.010.05418724
GAAAAAG133450.09.87895925
CGATCTA7400.09.7301538
AGTTGGT33600.09.64300330
AATTTGG54400.08.43736729
AAATTGG45800.08.31864729
AATTGGT26550.08.3052130
GGGGGAG272000.08.1175443
Produced by FastQC (version 0.11.5)