FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004955315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20029712
Sequences flagged as poor quality0
Sequence length44
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGCCTTGATC13295876.638073478041022TruSeq Adapter, Index 10 (97% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG383360.19139566260363605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1460050.037.727421
ATCGGAA1473750.037.6927222
TCGGAAG1482400.037.462873
ACACGTC1465050.037.44737613
ACGTCTG1468450.037.27167515
CACGTCT1470600.037.26489314
CGTCTGA1468800.037.25512716
CGGAAGA1490200.037.2502024
CACACGT1475050.037.19764712
GCACACG1481200.037.1008311
TCACTCG1467600.036.9648130
ACTCGCC1466950.036.88664232
AGAGCAC1499750.036.8111468
TCGCCTT1461250.036.810834
CACTCGC1473450.036.7883931
GAGCACA1498350.036.7833149
CGCCTTG1463650.036.67774635
AGCACAC1500000.036.67708210
CTTGATC1425800.036.60451538
TCTGAAC1494250.036.60088718
Produced by FastQC (version 0.11.5)