FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004955340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16628887
Sequences flagged as poor quality0
Sequence length36
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3580862.1533972778815564No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT571940.34394364457464893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCCG79500.026.4569643
GCTCTTC99750.021.4719371
CTCTTCC125650.017.3007162
TTCCGAT123500.017.0339745
CTTCCGA125550.016.6826274
TCCGATC160950.012.9505916
CCGATCT161750.012.2942747
CGATCTA26550.012.1459418
CCGATCG2701.2732926E-1111.10887057
AGGGGTT40100.010.810245530
CGATCTG38800.010.7079248
CGATCTT59000.09.8382128
GGGGGTT137000.09.60198730
GGGGAGA87400.09.3383522
CGATCTC75600.09.28498658
GGGGGAA122500.08.0344921
CGTTGGA29350.08.0231971
GGGGGGA312350.07.983256320
AAGGGGT32450.07.95054929
AACGGGT7700.07.7915988
Produced by FastQC (version 0.11.5)