FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004955351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17726402
Sequences flagged as poor quality0
Sequence length40
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACGCA904900.5104814840597658TruSeq Adapter, Index 3 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA311150.033.179321
ATCGGAA326300.033.0524062
TCGGAAG328600.032.805453
ACACGTC322000.032.75872413
CGTCTGA322900.032.62539316
ACGTCTG324700.032.4603315
CGGAAGA332650.032.370274
CACGTCT327450.032.21349314
TTACGCA99450.032.1538134
CACACGT329400.032.0382812
GCACACG329800.032.0303511
CTTACGC100800.031.8411433
ACTTACG101650.031.607832
AGAGCAC345500.030.6976788
AGCACAC348800.030.37321910
GAGCACA350050.030.2841859
GTCTGAA350800.030.09879717
TCTGAAC349500.030.07489818
GAAGAGC358050.030.0076266
CAGTCAC352150.029.7172327
Produced by FastQC (version 0.11.5)