FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004955388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13937837
Sequences flagged as poor quality0
Sequence length44
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTATCAAATC10868517.797845533707992TruSeq Adapter, Index 1 (97% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG258740.18563856070350082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1210500.037.4128272
GATCGGA1198350.037.3920441
TCGGAAG1220100.037.102973
ACACGTC1207150.036.99926413
CACACGT1212750.036.85661712
ACGTCTG1210150.036.8541615
CACGTCT1212500.036.8234714
CGTCTGA1211550.036.79916416
CGGAAGA1230350.036.7598884
GCACACG1218550.036.7153611
AGCACAC1225000.036.5494310
AGAGCAC1229400.036.5408368
CATTATC1202500.036.50337233
GAGCACA1228500.036.4993029
GTCACAT1210500.036.4159429
TCAAATC1168500.036.40485838
TCTGAAC1222800.036.3900318
CACATTA1210250.036.3889231
GTCTGAA1225550.036.367817
TTATCAA1203400.036.36735
Produced by FastQC (version 0.11.5)