FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004955398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4903567
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCTCATCTC998922.03712929791721TruSeq Adapter, Index 23 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCTCATGTC177070.36110447761802783TruSeq Adapter, Index 23 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCTCAGCTC125980.2569150171701539TruSeq Adapter, Index 23 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCTCATCGC118080.2408042961378931TruSeq Adapter, Index 23 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)