FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004955426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14029686
Sequences flagged as poor quality0
Sequence length40
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGTCTA1755761.2514606527900909TruSeq Adapter, Index 15 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA198350.033.226771
ATCGGAA216450.032.995582
TCGGAAG216200.032.954753
ACACGTC207000.032.7096513
CGTCTGA206450.032.6898616
CGGAAGA218500.032.615524
ACGTCTG208200.032.41497415
GCACACG211100.032.187111
CACGTCT210200.032.17125314
CACACGT211550.032.0463112
ACTTGTC218650.030.36947332
AGAGCAC228350.029.9415198
AGCACAC228000.029.90557510
GAAGAGC237750.029.6029036
CAGTCAC226300.029.59825527
GAGCACA231800.029.525229
CCAGTCA227550.029.43556226
TCTGAAC230550.029.4125918
GTCACTT230600.029.0685629
GTCTGAA234450.028.92342217
Produced by FastQC (version 0.11.5)