FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004955427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14029686
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1555341.1086064221251994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCCG55950.026.315463
GCTCTTC76500.019.6226371
TTCCGAT77600.018.7847885
CTTCCGA78500.018.6817174
CTCTTCC97100.015.7172672
CGATCTA18450.014.1444368
TCCGATC103500.014.0410866
CCGATCT103650.013.5868817
CGATCTT41900.011.4543058
GGGGGAA124450.010.88583921
CGATCTG23000.010.8246298
CGTTGGA25650.010.7575951
GGGGATA32450.010.49502222
GGGGAGA84550.010.18522222
CCGATCG1501.6485545E-49.9984397
GGGGAAA127100.09.89169422
GGGGGGA274550.09.89064720
CGATCTC49550.09.0805128
AAACGGG29650.08.9530717
ACGGGAT18300.08.769319
Produced by FastQC (version 0.11.5)