FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004955456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20500492
Sequences flagged as poor quality0
Sequence length40
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGTGCG11664485.689853687413941TruSeq Adapter, Index 14 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1267700.033.591131
ATCGGAA1298500.033.530242
ACACGTC1282550.033.30479413
TCGGAAG1307900.033.2903943
ACGTCTG1283500.033.21681215
CGTCTGA1285350.033.157116
CACGTCT1287550.033.1254514
CACACGT1291850.033.06766512
CGGAAGA1319450.033.0169374
GCACACG1301200.032.860111
AGAGCAC1310300.032.7979558
GAGCACA1311750.032.6994939
AGGTGCG1283100.032.6882534
AGCACAC1310950.032.6546110
TCTGAAC1306250.032.53418418
GTCTGAA1310750.032.4977117
CAGTCAC1304450.032.45005827
CCAGTCA1303250.032.4472526
GAAGAGC1341000.032.336766
CACAGGT1308000.032.32331531
Produced by FastQC (version 0.11.5)