FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004955457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20500492
Sequences flagged as poor quality0
Sequence length36
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5570272.7171396666967795No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT504110.24590141543920022No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGG219760.1071974272617457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGT122100.019.35539830
TCTTCCG49450.018.1267153
AGGTGGT72850.016.45714230
AAAGGTG75100.015.58362528
TTCCGAT78000.015.5254712
AAAGGGG132100.015.11781628
GGGAAAA335250.014.95789823
GGGAGAA157150.014.80915723
AAGGGGG136350.014.55913329
GGGGATA54450.014.21920122
GGAAAAA311850.014.1890924
CTTCCGA86650.014.0821411
GAAAGGG111450.013.4495527
GGGGAAA426950.013.21751122
GGGGAGA256100.013.18831422
GGGATAA46850.012.58681523
GGAGAAA155900.012.5215124
GGGGGAA494550.012.44227321
GAAAAAG166350.012.05948625
GCTCTTC77700.012.0579041
Produced by FastQC (version 0.11.5)