FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004955461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22970103
Sequences flagged as poor quality0
Sequence length36
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8496973.6991431862538886No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT513410.22351227593537565No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGG242500.10557201245462416No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGT138150.018.37680430
TCTTCCG50050.017.5504043
AGGTGGT76200.016.00870130
AAAGGTG84300.014.68322128
TTCCGAT58400.014.5838435
GGGAAAA378350.014.17760423
AAAGGGG158650.013.63704128
GGGGATA60100.013.55632822
GGAAAAA365150.013.36683424
GGGAGAA183000.013.29084123
AAGGGGG163500.013.2516529
CTTCCGA66400.013.0744184
GGGGAAA469250.012.6557622
TCCGATC67150.012.52761656
GGGATAA52800.012.44688623
GAAAGGG132350.012.24335627
GGGGAGA283450.012.04263922
GGGGGAA534450.012.00731921
GCTCTTC78300.011.640011
AAGGTGG87850.011.54583829
Produced by FastQC (version 0.11.5)