FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004955513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1893595
Sequences flagged as poor quality0
Sequence length46
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCTAGGATCTC19254710.168330609237984TruSeq Adapter, Index 13 (97% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43190.22808467491728696No Hit
CATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCTAGGATCTC21600.11406874226009256TruSeq Adapter, Index 27 (97% over 37bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCTAGGATCTC21030.11105859489489568TruSeq Adapter, Index 27 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCTAGGATGTC21030.11105859489489568TruSeq Adapter, Index 13 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)