FastQCFastQC Report
Thu 12 May 2022
EGAF00004955618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46035126
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCACAATCTCGTAT9040791.9638894873449462TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCACAATCGCGTAT3288490.7143436514108813TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCACAATCTCGTTT1554020.3375726613629775TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCACAATCTCGGAT1426590.30989162493005884TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCACAATCGCGTTT702570.15261606973770422TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCACAATCGCGGAT692010.150322169206184TruSeq Adapter, Index 23 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)