FastQCFastQC Report
Sat 4 Jun 2022
EGAF00004955620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56855447
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGCAATCTCGTAT10548521.8553226747122402TruSeq Adapter, Index 14 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGCAATCGCGTAT3669470.6454034210653554TruSeq Adapter, Index 14 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGCAATCTCGTTT2376080.41791598261464724TruSeq Adapter, Index 14 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGCAATCTCGGAT2031420.35729558154735813TruSeq Adapter, Index 14 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGCAATCGCGTTT1052560.18512913986939544TruSeq Adapter, Index 14 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGCAATCGCGGAT951630.16737710284821083TruSeq Adapter, Index 14 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGCAATCTCGGTT608140.10696248681326874TruSeq Adapter, Index 14 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)