FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004955627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14876225
Sequences flagged as poor quality0
Sequence length36-100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC78200.032.8045994
AGAGCGT77100.028.67619994
AAGAGCG144950.019.74143494
CGACCGT10100.018.695691
ATCGGAA291900.015.9096394
GATCGGA326650.014.99436194
TCGGAAG258700.014.82317494
CGGAAGA246200.014.093389594
AGATCGG390500.013.99197294
TAGGTCG7950.013.0073963
GACCGTT16150.012.5271872
ACCGTTG16900.011.7056533
CCGTATG21650.011.6294674
CAGATCG265700.010.92900194
CGACCTA15400.010.8021614
CGCAAAA60100.010.5477111
GCATTCG23400.010.1833273
GAAGAGC303100.010.13887794
GTCGTAA10201.8189894E-1210.1379825
GGAAGAG401150.09.90222594
Produced by FastQC (version 0.11.5)