FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004955628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20953959
Sequences flagged as poor quality0
Sequence length36-100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTCG41550.029.35255830-31
GTCGGGA51200.026.3864231
AGTCGTA49350.024.74691832-33
CGTCGGA34650.023.41209494
AACCGGA54000.023.08692672-73
CGCCATA74350.022.9505549
AACGCCA74000.022.9354637
AAACGCC76050.022.2570616
GAAACGC78400.021.9400255
TCAATCG29850.021.4560138
GTCGGGG155050.021.227961
GTCCGGA47000.021.1975771
GTCGTAA49500.021.15397832-33
GTCGGGT36900.020.9211751
AACGGCG30650.020.8959856
GTCGGTT46050.020.7287041
AGCACAC149100.020.64118294
TAGTCGT51450.020.2800630-31
GGCGGAT90200.020.182251
ACGCCAT85000.019.8597018
Produced by FastQC (version 0.11.5)