FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004955631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19488942
Sequences flagged as poor quality0
Sequence length36-100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC110900.030.56724794
AGAGCGT101850.028.92850594
AAGAGCG192950.021.3453294
ATCGGAA393350.016.30984994
CGACCTA19850.016.0941894
TCGGAAG355450.015.01846294
CGGAAGA340500.014.80945594
GATCGGA449700.014.41877394
AGATCGG530500.013.73556594
TACGCAC20650.013.493469
TAGGTCG11100.013.3771083
CTTGACG43800.011.813461
CGACCGT11050.011.8083481
CAGATCG361600.011.05402294
TTGACGA42700.010.9586952
CACGAAC26700.010.87687894
GAAGAGC410400.010.66596194
GACGACC34550.010.5484922
GCATTCG29300.09.9819883
CATTCGG24000.09.9365564
Produced by FastQC (version 0.11.5)