FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004955640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6967855
Sequences flagged as poor quality0
Sequence length36-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGTT17100.033.1979149
CTACGGT19750.028.7435138
TCTACGG23650.025.3477657
CGTTACG12700.024.18600732-33
AGTCTAC24600.024.1842755
CGAGTCT18650.023.1336843
GAGTCTA24700.022.9831714
AGCACAC54150.021.8512594
GTCTACG29100.021.5368926
GAGCACA72950.020.46971594
TACCCGA15250.020.3493560-61
AGAGCAC84500.019.81196494
CGACTTT24650.019.5292645
ACTTTCG25700.019.2615097
GAACGTA40750.018.7231355
GTTACGC16650.018.72149332-33
AACGTAT41250.018.496196
TTACCCG17000.018.49150558-59
ATTGTCG30550.018.4334818
CGCGACC23850.017.7649894
Produced by FastQC (version 0.11.5)