FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004955676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25697
Sequences flagged as poor quality0
Sequence length150-4058
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTTACGTATTGCTGGTGCTGCACCCACACTTACTTCAGGACGTATTAA2360.9183951434019535No Hit
GTTACGTATTGCTGGTGCTGCACCCACACTTACTTCAGGACGTATTAACC1240.482546600770518No Hit
CCAGTTACGTATTGCTGGTGCTGCACCCACACTTACTTCAGGACGTATTA570.2218157761606413No Hit
AGTTACGTATTGCTGGTGCTGCACCCACACTTACTTCAGGACGTATTAAC550.21403276647079422No Hit
GCGTTACGTATTGCTGGTGCTGCACCCACACTTACTTCAGGACGTATTAA480.1867922325563295No Hit
TGGTTACGTATTGCTGGTGCTGCACCCACACTTACTTCAGGACGTATTAA420.16344320348678834No Hit
CAGTTACGTATTGCTGGTGCTGCACCACACTTACTTCAGGACGTATTAAC390.15176868895201776No Hit
TTTACGTATTGCTGGTGCTGCACCCACACTTACTTCAGGACGTATTAACC310.1206366501926295No Hit
GGTTACGTATTGCTGGTGCTGCACCCACACTTACTTCAGGACGTATTAAC290.11285364050278243No Hit
CGGTTACGTATTGCTGGTGCTGCACCCACACTTACTTCAGGACGTATTAA290.11285364050278243No Hit
AGGTTACGTATTGCTGGTGCTGCACCCACACTTACTTCAGGACGTATTAA290.11285364050278243No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)