FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004955681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35273
Sequences flagged as poor quality0
Sequence length150-4218
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTTACGTATTGCTGGTGCTGAGAGGGTACTATGTGCCTCAGCACTTAA3430.9724151617384401No Hit
GTTACGTATTGCTGGTGCTGAGAGGGTACTATGTGCCTCAGCACTTAACC1720.48762509568224993No Hit
CAGTTACGTATTGCTGGTGCTGAGGGTACTATGTGCCTCAGCACTTAACC1130.32035834774473393No Hit
CCAGTTACGTATTGCTGGTGCTGAGAGGGTACTATGTGCCTCAGCACTTA760.215462251580529No Hit
GCGTTACGTATTGCTGGTGCTGAGAGGGTACTATGTGCCTCAGCACTTAA710.20128710345023107No Hit
AGTTACGTATTGCTGGTGCTGAGAGGGTACTATGTGCCTCAGCACTTAAC680.19278201457205227No Hit
TGGTTACGTATTGCTGGTGCTGAGAGGGTACTATGTGCCTCAGCACTTAA610.17293680718963514No Hit
CAGTTACGTATTGCTGGTGCTGAGAGGTACTATGTGCCTCAGCACTTAAC540.15309159980721798No Hit
TTTACGTATTGCTGGTGCTGAGAGGGTACTATGTGCCTCAGCACTTAACC470.13324639242480082No Hit
GCGTTACGTATTGCTGGTGCTGAGGGTACTATGTGCCTCAGCACTTAACC470.13324639242480082No Hit
AGGTTACGTATTGCTGGTGCTGAGAGGGTACTATGTGCCTCAGCACTTAA430.12190627392056248No Hit
AAAGTTACGTATTGCTGGTGCTGAGAGGGTACTATGTGCCTCAGCACTTA370.10489609616420492No Hit
GTTACGTATTGCTGGTGCTGAGGGTACTATGTGCCTCAGCACTTAACCTT360.10206106653814533No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)