FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004955682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16412
Sequences flagged as poor quality0
Sequence length150-1908
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTTACGTATTGCTGGTGCTGCATGTTCAACCAAGGCTTCTATGGTTAA2631.602485985864002No Hit
GTTACGTATTGCTGGTGCTGCATGTTCAACCAAGGCTTCTATGGTTAACC1140.6946136972946625No Hit
GCGTTACGTATTGCTGGTGCTGCATGTTCAACCAAGGCTTCTATGGTTAA640.38995856690226666No Hit
AGTTACGTATTGCTGGTGCTGCATGTTCAACCAAGGCTTCTATGGTTAAC540.32902754082378743No Hit
TTTACGTATTGCTGGTGCTGCATGTTCAACCAAGGCTTCTATGGTTAACC510.31074823300024373No Hit
TGGTTACGTATTGCTGGTGCTGCATGTTCAACCAAGGCTTCTATGGTTAA460.2802827199610042No Hit
CCAGTTACGTATTGCTGGTGCTGCATGTTCAACCAAGGCTTCTATGGTTA450.2741896173531562No Hit
TAAGTTACGTATTGCTGGTGCTGCATGTTCAACCAAGGCTTCTATGGTTA380.2315378990982208No Hit
GGTTACGTATTGCTGGTGCTGCATGTTCAACCAAGGCTTCTATGGTTAAC350.21325859127467708No Hit
AGGTTACGTATTGCTGGTGCTGCATGTTCAACCAAGGCTTCTATGGTTAA320.19497928345113333No Hit
CGTTACGTATTGCTGGTGCTGCATGTTCAACCAAGGCTTCTATGGTTAAC320.19497928345113333No Hit
TGCCATGTATTGCTGGTGCTGCATGTTCAACCAAGGCTTCTATGGTTAAC240.14623446258835No Hit
TGCCACGTATTGCTGGTGCTGCATGTTCAACCAAGGCTTCTATGGTTAAC220.13404825737265416No Hit
AAAGTTACGTATTGCTGGTGCTGCATGTTCAACCAAGGCTTCTATGGTTA190.1157689495491104No Hit
ATAGTTACGTATTGCTGGTGCTGCATGTTCAACCAAGGCTTCTATGGTTA180.10967584694126248No Hit
CGGTTACGTATTGCTGGTGCTGCATGTTCAACCAAGGCTTCTATGGTTAA180.10967584694126248No Hit
AAGTTACGTATTGCTGGTGCTGCATGTTCAACCAAGGCTTCTATGGTTAA170.10358274433341456No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)