FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004955687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22269
Sequences flagged as poor quality0
Sequence length150-3922
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTTACGTATTGCTGGTGCTGACCCTCCAGGAAAGTACCTCTGATTTAA2070.9295433113296512No Hit
GTTACGTATTGCTGGTGCTGACCCTCCAGGAAAGTACCTCTGATTTAACC1160.5209034981364228No Hit
GCGTTACGTATTGCTGGTGCTGACCCTCCAGGAAAGTACCTCTGATTTAA810.36373433921595044No Hit
CAGTTACGTATTGCTGGTGCTGACCTCCAGGAAAGTACCTCTGATTTAAC770.34577214962503927No Hit
CCAGTTACGTATTGCTGGTGCTGACCCTCCAGGAAAGTACCTCTGATTTA670.3008666756477615No Hit
GTTACGTATTGCTGGTGCTGACCTCCAGGAAAGTACCTCTGATTTAACCT550.24698010687502808No Hit
TGGTTACGTATTGCTGGTGCTGACCCTCCAGGAAAGTACCTCTGATTTAA450.20207463289775024No Hit
AGTTACGTATTGCTGGTGCTGACCCTCCAGGAAAGTACCTCTGATTTAAC400.17962189590911132No Hit
TTTACGTATTGCTGGTGCTGACCCTCCAGGAAAGTACCTCTGATTTAACC390.17513134851138354No Hit
AGGTTACGTATTGCTGGTGCTGACCCTCCAGGAAAGTACCTCTGATTTAA310.13920696932956125No Hit
CCAGTTACGTATTGCTGGTGCTGACCTCCAGGAAAGTACCTCTGATTTAA290.1302258745341057No Hit
GGTTACGTATTGCTGGTGCTGACCCTCCAGGAAAGTACCTCTGATTTAAC280.12573532713637792No Hit
TAAGTTACGTATTGCTGGTGCTGACCCTCCAGGAAAGTACCTCTGATTTA270.12124477973865014No Hit
GCGTTACGTATTGCTGGTGCTGACCTCCAGGAAAGTACCTCTGATTTAAC230.10328259014773901No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)