FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004955689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50982
Sequences flagged as poor quality0
Sequence length150-2227
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTTACGTATTGCTGGTGCTGGTTTGTCATACTCGTGTGCTCACCTTAA8641.6947157820407202No Hit
GTTACGTATTGCTGGTGCTGGTTTGTCATACTCGTGTGCTCACCTTAACC4750.9317013848024793No Hit
CCAGTTACGTATTGCTGGTGCTGGTTTGTCATACTCGTGTGCTCACCTTA2080.4079871327135067No Hit
AGTTACGTATTGCTGGTGCTGGTTTGTCATACTCGTGTGCTCACCTTAAC1970.3864108901180809No Hit
GCGTTACGTATTGCTGGTGCTGGTTTGTCATACTCGTGTGCTCACCTTAA1530.30010591973637757No Hit
CAGTTACGTATTGCTGGTGCTGGTTTGTCATACTCGTGCTCACCTTAACC1490.29226001333804086No Hit
TTTACGTATTGCTGGTGCTGGTTTGTCATACTCGTGTGCTCACCTTAACC1380.270683770742615No Hit
TGGTTACGTATTGCTGGTGCTGGTTTGTCATACTCGTGTGCTCACCTTAA1210.2373386685496842No Hit
GGTTACGTATTGCTGGTGCTGGTTTGTCATACTCGTGTGCTCACCTTAAC920.18045584716174337No Hit
CGTTACGTATTGCTGGTGCTGGTTTGTCATACTCGTGTGCTCACCTTAAC780.15299517476756502No Hit
AGGTTACGTATTGCTGGTGCTGGTTTGTCATACTCGTGTGCTCACCTTAA660.129457455572555No Hit
AAAGTTACGTATTGCTGGTGCTGGTTTGTCATACTCGTGTGCTCACCTTA640.12553450237338667No Hit
TAAGTTACGTATTGCTGGTGCTGGTTTGTCATACTCGTGTGCTCACCTTA630.12357302577380251No Hit
TGCCACGTATTGCTGGTGCTGGTTTGTCATACTCGTGTGCTCACCTTAAC620.12161154917421835No Hit
GTTACGTATTGCTGGTGCTGGTTTGTCATACTCGTGCTCACCTTAACCTT590.11572711937546584No Hit
TGCCATGTATTGCTGGTGCTGGTTTGTCATACTCGTGTGCTCACCTTAAC520.10199678317837667No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)