FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004955694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42879
Sequences flagged as poor quality0
Sequence length150-4315
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTTACGTATTGCTGGTGCTGACCACAGGAGGACGATACAGAGAATTAA5701.3293220457566641No Hit
GTTACGTATTGCTGGTGCTGACCACAGGAGGACGATACAGAGAATTAACC2340.5457216819422095No Hit
CCAGTTACGTATTGCTGGTGCTGACCACAGGAGGACGATACAGAGAATTA1710.3987966137269992No Hit
AGTTACGTATTGCTGGTGCTGACCACAGGAGGACGATACAGAGAATTAAC1540.3591501667482917No Hit
GCGTTACGTATTGCTGGTGCTGACCACAGGAGGACGATACAGAGAATTAA1470.3428251591688239No Hit
TGGTTACGTATTGCTGGTGCTGACCACAGGAGGACGATACAGAGAATTAA950.22155367429277736No Hit
CAGTTACGTATTGCTGGTGCTGACCACAGGAGGACGATACAGAATTAACC800.18657151519391776No Hit
GGTTACGTATTGCTGGTGCTGACCACAGGAGGACGATACAGAGAATTAAC690.1609179318547541No Hit
TTTACGTATTGCTGGTGCTGACCACAGGAGGACGATACAGAGAATTAACC650.1515893560950582No Hit
CGTTACGTATTGCTGGTGCTGACCACAGGAGGACGATACAGAGAATTAAC630.14692506821521026No Hit
AAAGTTACGTATTGCTGGTGCTGACCACAGGAGGACGATACAGAGAATTA520.12127148487604655No Hit
AGGTTACGTATTGCTGGTGCTGACCACAGGAGGACGATACAGAGAATTAA510.11893934093612259No Hit
CGGTTACGTATTGCTGGTGCTGACCACAGGAGGACGATACAGAGAATTAA450.10494647729657874No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)