FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004955697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20497
Sequences flagged as poor quality0
Sequence length151-1962
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTTACGTATTGCTGGTGCTGTCAATCAAGAAGGAAAGCAAGGTTTAAC2301.122115431526565No Hit
CAGTTACGTATTGCTGGTGCTGTCAATCAAGAAGGGAAAGCAAGGTTTAA970.4732399863394643No Hit
GTTACGTATTGCTGGTGCTGTCAATCAAGAAGGAAAGCAAGGTTTAACCT760.3707859686783432No Hit
CCAGTTACGTATTGCTGGTGCTGTCAATCAAGAAGGAAAGCAAGGTTTAA520.253695662779919No Hit
GTTACGTATTGCTGGTGCTGTCAATCAAGAAGGGAAAGCAAGGTTTAACC470.22930184905108064No Hit
AGTTACGTATTGCTGGTGCTGTCAATCAAGAAGGAAAGCAAGGTTTAACC430.20978679806800993No Hit
TGGTTACGTATTGCTGGTGCTGTCAATCAAGAAGGAAAGCAAGGTTTAAC430.20978679806800993No Hit
TTTACGTATTGCTGGTGCTGTCAATCAAGAAGGAAAGCAAGGTTTAACCT340.16587793335610088No Hit
GCGTTACGTATTGCTGGTGCTGTCAATCAAGAAGGAAAGCAAGGTTTAAC340.16587793335610088No Hit
CAGTTACGTATTGCTGGTGCTGTCAATCAAGAAGGAAGCAAGGTTTAACC330.16099917061033323No Hit
AGTTACGTATTGCTGGTGCTGTCAATCAAGAAGGGAAAGCAAGGTTTAAC330.16099917061033323No Hit
CCAGTTACGTATTGCTGGTGCTGTCAATCAAGAAGGGAAAGCAAGGTTTA280.13660535688149486No Hit
CAGTTACGTATTGCTGGTGCTGTCAATCAAGAAGGAAAGCAGGTTTAACC240.11709030589842416No Hit
TAAGTTACGTATTGCTGGTGCTGTCAATCAAGAAGGAAAGCAAGGTTTAA210.10245401766112114No Hit
GGTTACGTATTGCTGGTGCTGTCAATCAAGAAGGAAAGCAAGGTTTAACC210.10245401766112114No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)