FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004955698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32857
Sequences flagged as poor quality0
Sequence length150-2522
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTTACGTATTGCTGGTGCTGTCGATTCCGTTTGTAGTCGTCTGTTTAA4681.424354018930517No Hit
GTTACGTATTGCTGGTGCTGTCGATTCCGTTTGTAGTCGTCTGTTTAACC3871.1778312079617737No Hit
AGTTACGTATTGCTGGTGCTGTCGATTCCGTTTGTAGTCGTCTGTTTAAC1500.45652372401619135No Hit
CCAGTTACGTATTGCTGGTGCTGTCGATTCCGTTTGTAGTCGTCTGTTTA1340.4078278601211309No Hit
GCGTTACGTATTGCTGGTGCTGTCGATTCCGTTTGTAGTCGTCTGTTTAA1240.37739294518671823No Hit
TTTACGTATTGCTGGTGCTGTCGATTCCGTTTGTAGTCGTCTGTTTAACC1020.31043613233101014No Hit
TGGTTACGTATTGCTGGTGCTGTCGATTCCGTTTGTAGTCGTCTGTTTAA760.23130535350153694No Hit
GGTTACGTATTGCTGGTGCTGTCGATTCCGTTTGTAGTCGTCTGTTTAAC660.2008704385671242No Hit
GTTACGTATTGCTGGTGCTGTCGATTCGTTTGTAGTCGTCTGTTTAACCT590.17956599811303528No Hit
TGCCATGTATTGCTGGTGCTGTCGATTCCGTTTGTAGTCGTCTGTTTAAC580.176522506619594No Hit
CGTTACGTATTGCTGGTGCTGTCGATTCCGTTTGTAGTCGTCTGTTTAAC550.16739203213927017No Hit
CAGTTACGTATTGCTGGTGCTGTCGATTCGTTTGTAGTCGTCTGTTTAAC500.1521745746720638No Hit
GCCATGTATTGCTGGTGCTGTCGATTCCGTTTGTAGTCGTCTGTTTAACC460.1400006086982987No Hit
AAAGTTACGTATTGCTGGTGCTGTCGATTCCGTTTGTAGTCGTCTGTTTA440.13391362571141616No Hit
TGCCACGTATTGCTGGTGCTGTCGATTCCGTTTGTAGTCGTCTGTTTAAC390.11869616824420977No Hit
TAAGTTACGTATTGCTGGTGCTGTCGATTCCGTTTGTAGTCGTCTGTTTA390.11869616824420977No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)