FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004955703

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955703
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18143
Sequences flagged as poor quality0
Sequence length150-4146
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTTACGTATTGCTGGTGCTGGATCCAACAGAGATGCCTTCAGTGTTAA2001.1023535247753955No Hit
GTTACGTATTGCTGGTGCTGGATCCAACAGAGATGCCTTCAGTGTTAACC1190.6559003472413603No Hit
AGTTACGTATTGCTGGTGCTGGATCCAACAGAGATGCCTTCAGTGTTAAC640.3527531279281266No Hit
GCGTTACGTATTGCTGGTGCTGGATCCAACAGAGATGCCTTCAGTGTTAA480.2645648459460949No Hit
CCAGTTACGTATTGCTGGTGCTGGATCCAACAGAGATGCCTTCAGTGTTA470.25905307832221797No Hit
TTTACGTATTGCTGGTGCTGGATCCAACAGAGATGCCTTCAGTGTTAACC350.19291186683569422No Hit
CAGTTACGTATTGCTGGTGCTGGATCAACAGAGATGCCTTCAGTGTTAAC340.18740009921181724No Hit
GTTACGTATTGCTGGTGCTGGATCAACAGAGATGCCTTCAGTGTTAACCT310.1708647963401863No Hit
TGGTTACGTATTGCTGGTGCTGGATCCAACAGAGATGCCTTCAGTGTTAA310.1708647963401863No Hit
TGCCATGTATTGCTGGTGCTGGATCCAACAGAGATGCCTTCAGTGTTAAC240.13228242297304746No Hit
GGTTACGTATTGCTGGTGCTGGATCCAACAGAGATGCCTTCAGTGTTAAC240.13228242297304746No Hit
CGTTACGTATTGCTGGTGCTGGATCCAACAGAGATGCCTTCAGTGTTAAC230.12677065534917048No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)