FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004955706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14513
Sequences flagged as poor quality0
Sequence length150-2266
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTTACGTATTGCTGGTGCTGACGGTATGTCGAGTTCCAGGACTATTAA2271.564114931440777No Hit
GTTACGTATTGCTGGTGCTGACGGTATGTCGAGTTCCAGGACTATTAACC920.6339144215530903No Hit
CAGTTACGTATTGCTGGTGCTGACGGTATGTCGAGTTCAGGACTATTAAC780.5374491834906635No Hit
CCAGTTACGTATTGCTGGTGCTGACGGTATGTCGAGTTCCAGGACTATTA700.482326190312134No Hit
AGTTACGTATTGCTGGTGCTGACGGTATGTCGAGTTCCAGGACTATTAAC590.4065320746916558No Hit
GCGTTACGTATTGCTGGTGCTGACGGTATGTCGAGTTCCAGGACTATTAA510.35140908151312616No Hit
GTTACGTATTGCTGGTGCTGACGGTATGTCGAGTTCAGGACTATTAACCT420.2893957141872804No Hit
TGGTTACGTATTGCTGGTGCTGACGGTATGTCGAGTTCCAGGACTATTAA390.26872459174533175No Hit
GCGTTACGTATTGCTGGTGCTGACGGTATGTCGAGTTCAGGACTATTAAC350.241163095156067No Hit
TTTACGTATTGCTGGTGCTGACGGTATGTCGAGTTCCAGGACTATTAACC330.22738234686143455No Hit
CGTTACGTATTGCTGGTGCTGACGGTATGTCGAGTTCCAGGACTATTAAC280.19293047612485356No Hit
AAAGTTACGTATTGCTGGTGCTGACGGTATGTCGAGTTCCAGGACTATTA240.16536897953558877No Hit
ATAGTTACGTATTGCTGGTGCTGACGGTATGTCGAGTTCCAGGACTATTA220.15158823124095638No Hit
TGCCACGTATTGCTGGTGCTGACGGTATGTCGAGTTCCAGGACTATTAAC180.12402673465169159No Hit
AGTTACGTATTGCTGGTGCTGACGGTATGTCGAGTTCAGGACTATTAACC180.12402673465169159No Hit
CCAGTTACGTATTGCTGGTGCTGACGGTATGTCGAGTTCAGGACTATTAA180.12402673465169159No Hit
AGGTTACGTATTGCTGGTGCTGACGGTATGTCGAGTTCCAGGACTATTAA170.1171363605043754No Hit
GGTTACGTATTGCTGGTGCTGACGGTATGTCGAGTTCCAGGACTATTAAC160.11024598635705918No Hit
TGGTTACGTATTGCTGGTGCTGACGGTATGTCGAGTTCAGGACTATTAAC160.11024598635705918No Hit
TAAGTTACGTATTGCTGGTGCTGACGGTATGTCGAGTTCAGGACTATTAA160.11024598635705918No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)