FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004955715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24180
Sequences flagged as poor quality0
Sequence length150-2783
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTTACGTATTGCTGGTGCTGTGGCTTGATCTAGGTAAGGTCGAATTAA3071.269644334160463No Hit
GTTACGTATTGCTGGTGCTGTGGCTTGATCTAGGTAAGGTCGAATTAACC1370.5665839536807279No Hit
CCAGTTACGTATTGCTGGTGCTGTGGCTTGATCTAGGTAAGGTCGAATTA750.3101736972704715No Hit
GCGTTACGTATTGCTGGTGCTGTGGCTTGATCTAGGTAAGGTCGAATTAA730.30190239867659224No Hit
AGTTACGTATTGCTGGTGCTGTGGCTTGATCTAGGTAAGGTCGAATTAAC700.2894954507857734No Hit
CAGTTACGTATTGCTGGTGCTGTGGCTTGATCTAGGTAGGTCGAATTAAC460.1902398676592225No Hit
TTTACGTATTGCTGGTGCTGTGGCTTGATCTAGGTAAGGTCGAATTAACC440.18196856906534326No Hit
GGTTACGTATTGCTGGTGCTGTGGCTTGATCTAGGTAAGGTCGAATTAAC330.13647642679900746No Hit
TGGTTACGTATTGCTGGTGCTGTGGCTTGATCTAGGTAAGGTCGAATTAA320.13234077750206782No Hit
AAAGTTACGTATTGCTGGTGCTGTGGCTTGATCTAGGTAAGGTCGAATTA280.11579818031430934No Hit
TGCCATGTATTGCTGGTGCTGTGGCTTGATCTAGGTAAGGTCGAATTAAC260.10752688172043011No Hit
CGTTACGTATTGCTGGTGCTGTGGCTTGATCTAGGTAAGGTCGAATTAAC250.10339123242349049No Hit
CAGTTACGTATTGCTGGTGCTGGCTTGATCTAGGTAAGGTCGAATTAACC250.10339123242349049No Hit
TGCCACGTATTGCTGGTGCTGTGGCTTGATCTAGGTAAGGTCGAATTAAC250.10339123242349049No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)