FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004955720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18864
Sequences flagged as poor quality0
Sequence length150-2692
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTTACGTATTGCTGGTGCTGCGTCAACTGACAGTGGTTCGTACTTTAA3371.7864715860899067No Hit
GTTACGTATTGCTGGTGCTGCGTCAACTGACAGTGGTTCGTACTTTAACC1610.8534775233248516No Hit
CCAGTTACGTATTGCTGGTGCTGCGTCAACTGACAGTGGTTCGTACTTTA990.5248091603053435No Hit
GCGTTACGTATTGCTGGTGCTGCGTCAACTGACAGTGGTTCGTACTTTAA880.46649703138252757No Hit
TGGTTACGTATTGCTGGTGCTGCGTCAACTGACAGTGGTTCGTACTTTAA650.34457167090754875No Hit
AGTTACGTATTGCTGGTGCTGCGTCAACTGACAGTGGTTCGTACTTTAAC590.31276505513146735No Hit
TTTACGTATTGCTGGTGCTGCGTCAACTGACAGTGGTTCGTACTTTAACC470.24915182357930452No Hit
GGTTACGTATTGCTGGTGCTGCGTCAACTGACAGTGGTTCGTACTTTAAC400.21204410517387615No Hit
CGTTACGTATTGCTGGTGCTGCGTCAACTGACAGTGGTTCGTACTTTAAC390.20674300254452924No Hit
TGCCACGTATTGCTGGTGCTGCGTCAACTGACAGTGGTTCGTACTTTAAC360.19083969465648853No Hit
CGGTTACGTATTGCTGGTGCTGCGTCAACTGACAGTGGTTCGTACTTTAA360.19083969465648853No Hit
TAAGTTACGTATTGCTGGTGCTGCGTCAACTGACAGTGGTTCGTACTTTA280.1484308736217133No Hit
GCCATGTATTGCTGGTGCTGCGTCAACTGACAGTGGTTCGTACTTTAACC260.1378286683630195No Hit
TGCCATGTATTGCTGGTGCTGCGTCAACTGACAGTGGTTCGTACTTTAAC230.1219253604749788No Hit
AAAGTTACGTATTGCTGGTGCTGCGTCAACTGACAGTGGTTCGTACTTTA220.11662425784563189No Hit
AGGTTACGTATTGCTGGTGCTGCGTCAACTGACAGTGGTTCGTACTTTAA210.11132315521628498No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)