FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004955724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22187
Sequences flagged as poor quality0
Sequence length150-2224
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTTACGTATTGCTGGTGCTGGATGTAGAGGGTACGGTTTGAGGCTTAA2871.2935502771893452No Hit
CAGTTACGTATTGCTGGTGCTGGATGTAGAGGTACGGTTTGAGGCTTAAC880.39662865642042633No Hit
GTTACGTATTGCTGGTGCTGGATGTAGAGGGTACGGTTTGAGGCTTAACC840.37860008112858884No Hit
CCAGTTACGTATTGCTGGTGCTGGATGTAGAGGGTACGGTTTGAGGCTTA570.25690719790868527No Hit
AGTTACGTATTGCTGGTGCTGGATGTAGAGGGTACGGTTTGAGGCTTAAC460.20732861585613196No Hit
CAGTTACGTATTGCTGGTGCTGGATGTAGGGTACGGTTTGAGGCTTAACC460.20732861585613196No Hit
GTTACGTATTGCTGGTGCTGGATGTAGAGGTACGGTTTGAGGCTTAACCT450.20282147203317258No Hit
GCGTTACGTATTGCTGGTGCTGGATGTAGAGGGTACGGTTTGAGGCTTAA350.1577500338035787No Hit
TGGTTACGTATTGCTGGTGCTGGATGTAGAGGGTACGGTTTGAGGCTTAA300.1352143146887817No Hit
TTTACGTATTGCTGGTGCTGGATGTAGAGGGTACGGTTTGAGGCTTAACC240.10817145175102537No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)