FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004955727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22476
Sequences flagged as poor quality0
Sequence length150-2179
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTTACGTATTGCTGGTGCTGCTTTCGTTGTTGACTCGACGGTAGTTAA2621.165687844812244No Hit
GTTACGTATTGCTGGTGCTGCTTTCGTTGTTGACTCGACGGTAGTTAACC1590.7074212493326215No Hit
CCAGTTACGTATTGCTGGTGCTGCTTTCGTTGTTGACTCGACGGTAGTTA660.2936465563267485No Hit
GCGTTACGTATTGCTGGTGCTGCTTTCGTTGTTGACTCGACGGTAGTTAA640.2847481758319986No Hit
AGTTACGTATTGCTGGTGCTGCTTTCGTTGTTGACTCGACGGTAGTTAAC600.2669514148424987No Hit
TTTACGTATTGCTGGTGCTGCTTTCGTTGTTGACTCGACGGTAGTTAACC530.2358070831108738No Hit
CGTTACGTATTGCTGGTGCTGCTTTCGTTGTTGACTCGACGGTAGTTAAC430.19131518063712405No Hit
TGGTTACGTATTGCTGGTGCTGCTTTCGTTGTTGACTCGACGGTAGTTAA330.14682327816337426No Hit
GGTTACGTATTGCTGGTGCTGCTTTCGTTGTTGACTCGACGGTAGTTAAC290.12902651717387437No Hit
TGCCATGTATTGCTGGTGCTGCTTTCGTTGTTGACTCGACGGTAGTTAAC280.12457732692649937No Hit
GCCATGTATTGCTGGTGCTGCTTTCGTTGTTGACTCGACGGTAGTTAACC260.11567894643174942No Hit
CAGTTACGTATTGCTGGTGCTGCTTCGTTGTTGACTCGACGGTAGTTAAC230.1023313756896245No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)