FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004955737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31476
Sequences flagged as poor quality0
Sequence length150-1863
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTTACGTATTGCTGGTGCTGAGAACGACTTCCATACTCGTGTGATTAA3971.261278434362689No Hit
GTTACGTATTGCTGGTGCTGAGAACGACTTCCATACTCGTGTGATTAACC2350.7466005845723725No Hit
GCGTTACGTATTGCTGGTGCTGAGAACGACTTCCATACTCGTGTGATTAA1230.3907739229889439No Hit
CCAGTTACGTATTGCTGGTGCTGAGAACGACTTCCATACTCGTGTGATTA1150.36535773287584195No Hit
AGTTACGTATTGCTGGTGCTGAGAACGACTTCCATACTCGTGTGATTAAC840.2668699961875715No Hit
TGGTTACGTATTGCTGGTGCTGAGAACGACTTCCATACTCGTGTGATTAA810.2573389248951582No Hit
GGTTACGTATTGCTGGTGCTGAGAACGACTTCCATACTCGTGTGATTAAC810.2573389248951582No Hit
TTTACGTATTGCTGGTGCTGAGAACGACTTCCATACTCGTGTGATTAACC680.21603761596136736No Hit
TGCCATGTATTGCTGGTGCTGAGAACGACTTCCATACTCGTGTGATTAAC560.1779133307917143No Hit
TGCCACGTATTGCTGGTGCTGAGAACGACTTCCATACTCGTGTGATTAAC490.15567416444275003No Hit
CGTTACGTATTGCTGGTGCTGAGAACGACTTCCATACTCGTGTGATTAAC430.1366120218579235No Hit
AAAGTTACGTATTGCTGGTGCTGAGAACGACTTCCATACTCGTGTGATTA370.11754987927309697No Hit
AGGTTACGTATTGCTGGTGCTGAGAACGACTTCCATACTCGTGTGATTAA360.1143728555089592No Hit
CAGTTACGTATTGCTGGTGCTGAGAACGACTTCATACTCGTGTGATTAAC340.10801880798068368No Hit
CGGTTACGTATTGCTGGTGCTGAGAACGACTTCCATACTCGTGTGATTAA330.10484178421654594No Hit
CAGTTACGTATTGCTGGTGCTGAACGACTTCCATACTCGTGTGATTAACC330.10484178421654594No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)