FastQCFastQC Report
Wed 8 May 2024
EGAF00004955738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9441
Total Bases6.1 Mbp
Sequences flagged as poor quality0
Sequence length152-2034
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTACGTATTGCTGGTGCTGCGACGGTTAGATTCACCTCTTACATTAACC880.9321046499311514No Hit
CAGTTACGTATTGCTGGTGCTGCGACGGTTAGATTCACCTCTTACATTAA750.7944073721004131No Hit
TTTACGTATTGCTGGTGCTGCGACGGTTAGATTCACCTCTTACATTAACC350.3707234403135261No Hit
AGTTACGTATTGCTGGTGCTGCGACGGTTAGATTCACCTCTTACATTAAC300.31776294884016526No Hit
CCAGTTACGTATTGCTGGTGCTGCGACGGTTAGATTCACCTCTTACATTA260.2753945556614765No Hit
TGCCATGTATTGCTGGTGCTGCGACGGTTAGATTCACCTCTTACATTAAC250.26480245736680436No Hit
GCCATGTATTGCTGGTGCTGCGACGGTTAGATTCACCTCTTACATTAACC250.26480245736680436No Hit
GCGTTACGTATTGCTGGTGCTGCGACGGTTAGATTCACCTCTTACATTAA200.2118419658934435No Hit
GGTTACGTATTGCTGGTGCTGCGACGGTTAGATTCACCTCTTACATTAAC190.2012498675987713No Hit
GTTACGTATTGCTGGTGCTGCGACGGTTGATTCACCTCTTACATTAACCT140.14828937612541043No Hit
GTTGCGTATTGCTGGTGCTGCGACGGTTAGATTCACCTCTTACATTAACC120.1271051795360661No Hit
TGGTTACGTATTGCTGGTGCTGCGACGGTTAGATTCACCTCTTACATTAA110.11651308124139392No Hit
TGCCACGTATTGCTGGTGCTGCGACGGTTAGATTCACCTCTTACATTAAC110.11651308124139392No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)