FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004955741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31225
Sequences flagged as poor quality0
Sequence length150-3997
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTTACGTATTGCTGGTGCTGGGACGAAGAACTCAAGTCAAAGGCTTAA5111.6365092073658927No Hit
GTTACGTATTGCTGGTGCTGGGACGAAGAACTCAAGTCAAAGGCTTAACC1790.5732586068855084No Hit
CCAGTTACGTATTGCTGGTGCTGGGACGAAGAACTCAAGTCAAAGGCTTA1400.44835868694955966No Hit
CAGTTACGTATTGCTGGTGCTGGACGAAGAACTCAAGTCAAAGGCTTAAC1170.37469975980784626No Hit
GCGTTACGTATTGCTGGTGCTGGGACGAAGAACTCAAGTCAAAGGCTTAA1080.3458767013610889No Hit
AGTTACGTATTGCTGGTGCTGGGACGAAGAACTCAAGTCAAAGGCTTAAC990.31705364291433147No Hit
TGGTTACGTATTGCTGGTGCTGGGACGAAGAACTCAAGTCAAAGGCTTAA940.3010408326661329No Hit
CAGTTACGTATTGCTGGTGCTGGGACGAAGAACTCAAGTCAAGGCTTAAC650.2081665332265813No Hit
AGGTTACGTATTGCTGGTGCTGGGACGAAGAACTCAAGTCAAAGGCTTAA480.15372297838270615No Hit
TTTACGTATTGCTGGTGCTGGGACGAAGAACTCAAGTCAAAGGCTTAACC400.12810248198558846No Hit
GTTACGTATTGCTGGTGCTGGACGAAGAACTCAAGTCAAAGGCTTAACCT360.11529223378702962No Hit
TAAGTTACGTATTGCTGGTGCTGGGACGAAGAACTCAAGTCAAAGGCTTA360.11529223378702962No Hit
AAAGTTACGTATTGCTGGTGCTGGGACGAAGAACTCAAGTCAAAGGCTTA360.11529223378702962No Hit
CGTTACGTATTGCTGGTGCTGGGACGAAGAACTCAAGTCAAAGGCTTAAC360.11529223378702962No Hit
CCAGTTACGTATTGCTGGTGCTGGACGAAGAACTCAAGTCAAAGGCTTAA350.11208967173738991No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)