FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004955742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49575
Sequences flagged as poor quality0
Sequence length150-2962
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTTACGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAA7891.5915279878971254No Hit
GTTACGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACC4010.8088754412506304No Hit
CCAGTTACGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTA1990.4014120020171458No Hit
AGTTACGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAAC1500.30257186081694404No Hit
TTTACGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACC1460.2945032778618255No Hit
GCGTTACGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAA1290.26021180030257185No Hit
CAGTTACGTATTGCTGGTGCTGAAGAAGTTGTCGGTGTCTTTGTGTTAAC1270.2561775088250126No Hit
TGGTTACGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAA1180.238023197175996No Hit
GTTACGTATTGCTGGTGCTGAAGAAGTTGTCGGTGTCTTTGTGTTAACCT1070.21583459404942007No Hit
AAAGTTACGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTA960.19364599092284418No Hit
CGTTACGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAAC920.18557740796772568No Hit
GGTTACGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAAC830.16742309631870902No Hit
TGCCACGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAAC780.1573373676248109No Hit
TAAGTTACGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTA740.1492687846696924No Hit
TGCCATGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAAC740.1492687846696924No Hit
CAGTTACGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTGTGTTAAC720.14523449319213314No Hit
AGGTTACGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAA660.13313161875945537No Hit
CAGTTACGTATTGCTGGTGCTGAAAGTTGTCGGTGTCTTTGTGTTAACCT660.13313161875945537No Hit
TTTACGTATTGCTGGTGCTGAAGAAGTTGTCGGTGTCTTTGTGTTAACCT570.11497730711043873No Hit
AAGTTACGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAA560.11296016137165911No Hit
CGGTTACGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAA560.11296016137165911No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)