FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004955745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30405
Sequences flagged as poor quality0
Sequence length150-2559
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTTACGTATTGCTGGTGCTGTTCAGGAACAAACCAAGTTACGTTTAAC3141.032724880776188No Hit
GTTACGTATTGCTGGTGCTGTTCAGGAACAAACCAAGTTACGTTTAACCT2520.8288110508140107No Hit
CAGTTACGTATTGCTGGTGCTGTTCAGGGAACAAACCAAGTTACGTTTAA1530.5032067094227922No Hit
GTTACGTATTGCTGGTGCTGTTCAGGGAACAAACCAAGTTACGTTTAACC1320.434139121854958No Hit
AGTTACGTATTGCTGGTGCTGTTCAGGAACAAACCAAGTTACGTTTAACC1170.38480513073507644No Hit
GCGTTACGTATTGCTGGTGCTGTTCAGGAACAAACCAAGTTACGTTTAAC960.31573754316724223No Hit
TGGTTACGTATTGCTGGTGCTGTTCAGGAACAAACCAAGTTACGTTTAAC820.26969248478868607No Hit
CCAGTTACGTATTGCTGGTGCTGTTCAGGAACAAACCAAGTTACGTTTAA690.22693635915145535No Hit
GGTTACGTATTGCTGGTGCTGTTCAGGAACAAACCAAGTTACGTTTAACC690.22693635915145535No Hit
AGGTTACGTATTGCTGGTGCTGTTCAGGAACAAACCAAGTTACGTTTAAC630.20720276270350269No Hit
TTTACGTATTGCTGGTGCTGTTCAGGAACAAACCAAGTTACGTTTAACCT610.20062489722085186No Hit
AGTTACGTATTGCTGGTGCTGTTCAGGGAACAAACCAAGTTACGTTTAAC570.18746916625555007No Hit
GCGTTACGTATTGCTGGTGCTGTTCAGGGAACAAACCAAGTTACGTTTAA550.1808913007728992No Hit
TGGTTACGTATTGCTGGTGCTGTTCAGGGAACAAACCAAGTTACGTTTAA460.15129090610097023No Hit
AGGTTACGTATTGCTGGTGCTGTTCAGGGAACAAACCAAGTTACGTTTAA430.14142410787699392No Hit
GGTTACGTATTGCTGGTGCTGTTCAGGGAACAAACCAAGTTACGTTTAAC430.14142410787699392No Hit
TAAGTTACGTATTGCTGGTGCTGTTCAGGAACAAACCAAGTTACGTTTAA410.13484624239434304No Hit
GCCATGTATTGCTGGTGCTGTTCAGGAACAAACCAAGTTACGTTTAACCT370.12169051142904128No Hit
CCAGTTACGTATTGCTGGTGCTGTTCAGGGAACAAACCAAGTTACGTTTA350.1151126459463904No Hit
TTTACGTATTGCTGGTGCTGTTCAGGGAACAAACCAAGTTACGTTTAACC350.1151126459463904No Hit
CGTTACGTATTGCTGGTGCTGTTCAGGAACAAACCAAGTTACGTTTAACC330.1085347804637395No Hit
CAGTTACGTATTGCTGGTGCTGTTCAGGAACAAACCAGTTACGTTTAACC330.1085347804637395No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)