FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004955769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25463
Sequences flagged as poor quality0
Sequence length151-2186
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTTACGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAA3101.2174527746141461No Hit
GTTACGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACC2591.0171621568550444No Hit
AGTTACGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAAC950.3730903664140125No Hit
GTTACGTATTGCTGGTGCTGAAGAAGTTGTCGGTGTCTTTGTGTTAACCT740.2906177591014413No Hit
TTTACGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACC720.2827632250716726No Hit
GCGTTACGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAA510.20029061775910145No Hit
GGTTACGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAAC500.1963633507442171No Hit
CAGTTACGTATTGCTGGTGCTGAAGAAGTTGTCGGTGTCTTTGTGTTAAC460.18065428268467973No Hit
TGCCATGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAAC430.1688724816400267No Hit
TGGTTACGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAA400.15709068059537368No Hit
CCAGTTACGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTA370.14530887955072064No Hit
CAGTTACGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTGTGTTAAC350.13745434552095195No Hit
CGTTACGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAAC340.13352707850606763No Hit
CAGTTACGTATTGCTGGTGCTGAAAGTTGTCGGTGTCTTTGTGTTAACCT290.1138907434316459No Hit
TTTACGTATTGCTGGTGCTGAAGAAGTTGTCGGTGTCTTTGTGTTAACCT290.1138907434316459No Hit
AAAGTTACGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTA280.10996347641676159No Hit
TGCCACGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAAC260.10210894238699289No Hit
GCCATGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACC260.10210894238699289No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)