FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004955771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105166
Sequences flagged as poor quality0
Sequence length150-2596
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTTACGTATTGCTGGTGCTGTCGATTCCGTTTGTAGTCGTCTGTTTAA17981.709678032824297No Hit
GTTACGTATTGCTGGTGCTGTCGATTCCGTTTGTAGTCGTCTGTTTAACC9800.931860106878649No Hit
GCGTTACGTATTGCTGGTGCTGTCGATTCCGTTTGTAGTCGTCTGTTTAA5700.5420002662457448No Hit
CCAGTTACGTATTGCTGGTGCTGTCGATTCCGTTTGTAGTCGTCTGTTTA5310.5049160375026149No Hit
AGTTACGTATTGCTGGTGCTGTCGATTCCGTTTGTAGTCGTCTGTTTAAC4150.39461422893330544No Hit
TGGTTACGTATTGCTGGTGCTGTCGATTCCGTTTGTAGTCGTCTGTTTAA3490.33185630336800864No Hit
TTTACGTATTGCTGGTGCTGTCGATTCCGTTTGTAGTCGTCTGTTTAACC3020.28716505334423675No Hit
GGTTACGTATTGCTGGTGCTGTCGATTCCGTTTGTAGTCGTCTGTTTAAC2530.24057204800030427No Hit
CGTTACGTATTGCTGGTGCTGTCGATTCCGTTTGTAGTCGTCTGTTTAAC2530.24057204800030427No Hit
AAAGTTACGTATTGCTGGTGCTGTCGATTCCGTTTGTAGTCGTCTGTTTA2440.232014149059582No Hit
CAGTTACGTATTGCTGGTGCTGTCGATTCGTTTGTAGTCGTCTGTTTAAC1890.17971587775516804No Hit
TAAGTTACGTATTGCTGGTGCTGTCGATTCCGTTTGTAGTCGTCTGTTTA1810.17210885647452598No Hit
TGCCATGTATTGCTGGTGCTGTCGATTCCGTTTGTAGTCGTCTGTTTAAC1680.1597474468934827No Hit
TGCCACGTATTGCTGGTGCTGTCGATTCCGTTTGTAGTCGTCTGTTTAAC1630.15499305859308143No Hit
AGGTTACGTATTGCTGGTGCTGTCGATTCCGTTTGTAGTCGTCTGTTTAA1550.14738603731243938No Hit
CGGTTACGTATTGCTGGTGCTGTCGATTCCGTTTGTAGTCGTCTGTTTAA1440.1369263830515566No Hit
GTTACGTATTGCTGGTGCTGTCGATTCGTTTGTAGTCGTCTGTTTAACCT1330.12646672879067378No Hit
CAAGTTACGTATTGCTGGTGCTGTCGATTCCGTTTGTAGTCGTCTGTTTA1110.10554742026890819No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)