FastQCFastQC Report
Thu 16 May 2024
EGAF00004955840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63966135
Total Bases6.4 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG993400.1553009260290621No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT954180.14916955667244863Illumina Single End PCR Primer 1 (100% over 50bp)
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT653670.10219001038596438No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)