FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004955921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17499083
Sequences flagged as poor quality0
Sequence length15-41
%GC64

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACCAG156550.087.9287633
TACCAGA159750.084.89210534
TTACTAC145650.081.4572930
ACCAGAC168000.081.3996635
ACCTTAC149400.081.2675227
CACCTTA148550.081.2130626
CTTACTA145900.080.73807529
TACTACC148500.080.69733431
ACTACCA150150.080.2196432
CCTTACT151650.079.7965828
CCACCTT159300.076.36565425
CTCCACC183850.067.6003323
CGTACGA2700.066.8026129
ACTCCAC166450.066.4560522
TTAGACG6050.066.1447634
TATAGGC21300.063.7850535
TCCACCT193950.062.21685824
ACGAGTA9300.061.18202627
AGTATAG22950.059.6134333
GTATAGG23350.058.60824234
Produced by FastQC (version 0.11.5)