FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004955926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23115180
Sequences flagged as poor quality0
Sequence length15-42
%GC63

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTTAC34150.0107.64712527
CACCTTA34250.0106.9246326
CTTACTA34600.0105.90884429
TTACTAC34750.0105.67547630
TACTACC37700.097.8183731
CCTTACT38300.095.8252928
ACTACCA40200.092.0139732
CCACCTT44750.082.3105125
CTACCAG47050.079.3173633
TACCAGA51250.073.9174734
TCCACCT50050.073.4844724
ACGAGTA7250.071.196127
TTCGGAT45300.070.59531428
CGGATCA47000.067.94704430
TCGGATC48350.066.15346529
CCAGACA59600.066.0621636
TCGCAGT39600.065.9679927
ACCAGAC58050.065.2595335
ATCATGT50100.064.4808333
TGGTCCT43750.064.09144635
Produced by FastQC (version 0.11.5)