FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004955929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26148873
Sequences flagged as poor quality0
Sequence length15-42
%GC65

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTAC102650.064.98430
CTTACTA103700.064.3001929
CACCTTA103850.064.0651226
ACCTTAC104700.063.7597627
CCTTACT107800.062.0425428
TACCAGA110000.061.73017534
TACTACC109800.060.59570331
ACTACCA110150.060.4827632
CTACCAG115850.057.77821433
CCACCTT119050.055.88800425
TATAGGC23750.053.9804735
TCCACCT126550.052.4540524
ACTCCAC116700.052.0820522
GTATAGG24700.051.904334
ATAGGCG25350.050.5734236
AGTATAG25700.050.33938233
CCAGACA144950.048.08008236
CTATCTA6750.047.48282235
ATCACCT60700.047.2057231
ATCGTCG60550.046.90413728
Produced by FastQC (version 0.11.5)