FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004955931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34502410
Sequences flagged as poor quality0
Sequence length15-42
%GC65

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGTGTGGTCAGGCGGCTCGGACTG471190.13656727167754368No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCTTA108350.0103.8271326
TTACTAC110150.0102.1869730
ACCTTAC110350.0102.08372527
CTTACTA111100.0101.2785629
TACTACC116050.097.0714431
CCTTACT117050.096.2347628
ACTACCA124050.091.0202932
ACTATTA24000.090.72877536
CCACCTT129300.087.11500525
TACCAGA137600.083.06962634
CTACCAG137500.082.5823933
TCCACCT137650.081.67724624
CCAGACA144000.080.3001836
ACCAGAC148450.076.9603535
ACGAGTA11700.076.4830727
TACTATT28650.076.20582635
TTAGACG11600.075.1854634
TGTACTA29550.072.6416533
GTACTAT29950.072.51134534
CTATCTA8350.069.86439535
Produced by FastQC (version 0.11.5)