FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004955933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25190479
Sequences flagged as poor quality0
Sequence length15-42
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCTTA374650.063.68011526
ACCTTAC376550.063.4242927
TTACTAC375200.063.39542830
CTTACTA377300.063.01042629
CCTTACT383050.062.41402428
ACTACCA382650.062.0226932
CTACCAG389050.061.31270633
CCACCTT389150.061.23566425
TACTACC392700.060.55479431
TACCAGA402800.060.14892234
TCCACCT395700.060.09929724
CCAGACA409500.059.66129336
ACCAGAC406600.059.62211235
CTCCACC423000.056.0590823
ACTCCAC386250.055.39201722
ATAGGCG42850.039.60421436
TTAGACG7400.039.28679734
ACTATTA12050.039.27620736
GTATAGG44700.038.11561234
AGTATAG44350.037.8515733
Produced by FastQC (version 0.11.5)